● Recognizes all proteins which contain either an N or C-terminal HHHHHHGS epitope tag. It has been shown to recognise HHHHHH alone.
Overview
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Product name
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Anti-6×His tag antibody [HS1] - Pro Marketing
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Description
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Mouse monoclonal [HS1] to 6×His tag
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Specificity
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Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
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Tested applications
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WB, ICC/IF, IP
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Immunogen
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6× His synthetic peptide
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Properties
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage buffer
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pH: 7.2, Preservative: 0.1% Sodium azide, Constituent: PBS.
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Concentration
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100 µg at 1 mg/ml
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Purity
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IgG fraction
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Clonality
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Monoclonal
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Clone number
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HS1
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Isotype
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IgG2a
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Applications
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Target
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Relevance
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The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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Cellular localization
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Depends upon the localization of the parent protein tagged with hexahistidine.
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Alternative names
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6 His epitope tag antibody, Hexa His tag antibody, HHHHHH epitope tag antibody, HHHHHH tag antibody, His tag antibody, Polyhistidine Tag antibody, etc.
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Anti-6×His tag antibody [HIS-1] - Pro Marketing images
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