标签抗体
Anti-GFP tag antibody [GF9] - Pro Marketing (PMA106)
产品优势
● Assayed by ELISA for direct binding of antigen recognizes wild type and recombinant GFP. Well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. Shows no reactivity against red fluorescence protein (RFP). This antibody is known to cross react with the wild type (wt), recombinant (rGFP) and enhanced (eGFP) forms.
产品描述
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Overview
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Product name
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Anti-GFP tag antibody [GF9] - Pro Marketing
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Description
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Mouse monoclonal [GF9] to GFP tag
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Reactivity
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Green fluorescent protein (GFP), Yellow fluorescent protein(YFP), Cyan fluorescent protein(CFP).
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Tested applications
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WB, IP
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Immunogen
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A Green Fluorescent Protein (GFP) fusion protein
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Properties
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage buffer
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pH: 7.2, Preservative: 0.01% Sodium Azide, Constituent: PBS.
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Concentration
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100 µg at 1 mg/ml
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Purity
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Protein A purified
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Clonality
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Monoclonal
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Clone number
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GF9
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Isotype
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IgG2a
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Applications
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Target
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Relevance
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Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
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Alternative names
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GFP antibody, Green fluorescent protein antibody, yfp antibody, etc.
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Anti-GFP tag antibody [GF9] - Pro Marketing images
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