● 高质量的GAPDH小鼠单抗
● WB稀释比高达1/5000 - 1/20000
● 可用于WB, IHC, ICC/IF, FC, IP, ELISA等实验
Overview
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Product name
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Anti GAPDH antibody [mPro4] - Loading Control
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Description
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Mouse monoclonal [mPro4] to GAPDH
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Tested applications
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WB, IHC, ICC/IF, FC, IP, ELISA
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Species reactivity
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Reacts with: Human, Mouse, Rat, Zebrafish, Yeast. Other species are not tested, Please decide the specificity by homology.
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Immunogen
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Full length native protein from human erythrocytes.
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Positive control
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This antibody gave a positive signal within WB in the following whole cell lysates: HeLa; HEK293; HepG2; NIH3T3.
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Properties
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Form
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Liquid
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Storage instructions
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Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage buffer
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pH: 7.40, Preservative: 0.02% Sodium azide, Constituent: PBS
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Concentration
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100 µg at 1 mg/ml
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Purity
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IgG fraction
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Clonality
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Monoclonal
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Clone number
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mPro4
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Isotype
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IgG2b
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Applications
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Target
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Function
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Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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Alternative names
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G3P_HUMAN antibody, G3PD antibody, G3PDH antibody, GAPD antibody, GAPDH antibody, etc.
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Anti GAPDH antibody [mPro4] - Loading Control images
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