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Anti GAPDH antibody [mPro4] - Loading Control (PMC203)

货号
PMC203
规格
50μl/100μl/200μl
价格
¥800.00/1200.00/2100.00
 
 
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产品优势

● 高质量的GAPDH小鼠单抗
● WB稀释比高达1/5000 - 1/20000
● 可用于WB, IHC, ICC/IF, FC, IP, ELISA等实验

产品描述

Overview                                                      
Product name
Anti GAPDH antibody [mPro4] - Loading Control                                        
Description
Mouse monoclonal [mPro4] to GAPDH
Tested applications
WB, IHC, ICC/IF, FC, IP, ELISA
Species reactivity
Reacts with: Human, Mouse, Rat, Zebrafish, Yeast. Other species are not tested, Please decide the specificity by homology.
Immunogen
Full length native protein from human erythrocytes.
Positive control
This antibody gave a positive signal within WB in the following whole cell lysates: HeLa; HEK293; HepG2; NIH3T3.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40, Preservative: 0.02% Sodium azide, Constituent: PBS
Concentration
100 µg at 1 mg/ml
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mPro4
Isotype
IgG2b
Applications
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1/5000 - 1/20000.
IHC
1/20 - 1/200.
IF
1/20 - 1/200.
IP
1/5000 - 1/20000.
FC
Use at an assay dependent concentration.
Target
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Alternative names
G3P_HUMAN antibody, G3PD antibody, G3PDH antibody, GAPD antibody, GAPDH antibody, etc.
Anti GAPDH antibody [mPro4] - Loading Control images
 
                
 
All lanes : Anti GAPDH antibody [mPro4] - Loading Control (PMC203) at 1/20000 dilution
 
Lane 1 : HeLa Whole Cell Lysate.
Lane 2 : NIH3T3 Cell.
 
Lysates/proteins at 10 µg per lane.
Western blot - Anti GAPDH antibody [mPro4] - Loading Control (PMC203)

 

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